Chen, Shujing; Wang, Yuan; Liang, Chunxiao; Li, Jin; Li, Yuhong; Wu, Qi; Liu, Zhijian; Pang, Xiaoli; Chang, Yan-xu published the artcile< Buyang Huanwu Decoction ameliorates atherosclerosis by regulating TGF-ç?Smad2 pathway to promote the differentiation of regulatory T cells>, COA of Formula: C19H34O2, the main research area is atherosclerosis Buyang Huanwu decoction Smad2 regulatory T cell; Atherosclerosis; Buyang Huanwu decoction; Regulatory T cells; TGF-ç?Smad2 pathway.
This study aims to elucidate the anti-atherosclerosis mechanism of BYHWD, which may promote the differentiation of regulatory T cells by regulating the TGF-ç?Smad2 pathway. ApoE-/- mice were fed a high-fat diet for 12 wk, then drugs group were given BYHWD with intragastric administration once a day for 4 wk. The effect of BYHWD on lipid content in peripheral blood and plaque was evaluated by blood lipid test and oil red O staining. The number of Tregs in peripheral blood was tested by flow cytometry, and that in the spleen was evaluated by immunohistochem. methods. Gene and protein expression relating with Tregs differentiation pathway in mice were checked by RT-PCR and Western blot experiments The chem. compositions of BYHWD and rat drug-loaded serum were analyzed by ultra-high performance liquid chromatograph and liquid chromatog.-tandem mass spectrometry. BYHWD significantly reduced plaque area and cholesterol accumulation, increased the number of Tregs in spleen and peripheral blood of ApoE-/- AS mice, raised the proportion of Tregs in CD4+T cells, and regulated the levels of inflammatory factors. The mRNA levels of Foxp3/TGF-ç?Smad2 were enhanced via BYHWD in vitro. BYHWD regulates TGF-ç?Smad2 signaling pathway to promotes the peripheral differentiation of Tregs, increases the number of Tregs, restores the immune balance between CD4+T cells, regulates lipid metabolism, inhibits inflammatory reaction and possesses the potential of enhancing plaque stability.
Journal of Ethnopharmacology published new progress about Angelica sinensis. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, COA of Formula: C19H34O2.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics