Amouzadeh Tabrizi, Mahmoud; Shamsipur, Mojtaba; Saber, Reza; Sarkar, Saeed; Besharati, Maryam published the artcile< An electrochemical aptamer-based assay for femtomolar determination of insulin using a screen printed electrode modified with mesoporous carbon and 1,3,6,8-pyrenetetrasulfonate>, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is electrochem aptamer assay femtomolar insulin electrode carbon pyrenetetrasulfonate; 1,3,6,8-Pyrenetetrasulfonate; Differential pulse voltammetry; Insulin; Methylene Blue; Modified electrode; Ordered mesoporous carbon; Screen printed electrode.
The authors describe an electrochem. method for aptamer-based determination of insulin at femtomolar concentrations The surface of a screen printed electrode was modified with ordered mesoporous carbon that was chem. modified with 1,3,6,8-pyrenetetrasulfonate (TPS). The amino-terminated aptamer was then covalently linked to TPS via reactive sulfonyl chloride groups. Subsequently, the redox probe Methylene Blue (MB) was interacted into the aptamer. The MB-modified binds to insulin and this results in the release of MB and a decreased signal as obtained by differential pulse voltammetry, best at a working voltage of -0.3 V (vs. silver pseudo-reference electrode). Insulin can be quantified by this method in the 1.0 f. to 10.0 pM concentration range, with a 0.18 f. limit of detection (at 3delta/slope). The assay was applied to the determination of insulin in spiked human serum samples. The method is highly sensitive, selective, stable, and has a wide anal. range.
Microchimica Acta published new progress about Aptamers. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics