Xu, Meng; Chigumira, Tafadzwa; Chen, Ziheng; Tones, Jason; Zhao, Rongwei; Dahl, Kris Noel; Chenoweth, David M.; Zhang, Huaiying published the artcile< CRISPR Cas13-based tools to track and manipulate endogenous telomeric repeat-containing RNAs in live cells>, Application In Synthesis of 112-63-0, the main research area is U2OS cell TERRA telomere CRISPR Cas13; CRISPR Cas13; RNA imaging; TERRA; chemical dimerizer; lncRNA; telomere.
TERRA, TElomeric Repeat-containing RNA, is a long non-coding RNA transcribed from telomeres. Emerging evidence indicates that TERRA regulates telomere maintenance and chromosome end protection in normal and cancerous cells. However, the mechanism of how TERRA contributes to telomere functions is still unclear, partially owing to the shortage of approaches to track and manipulate endogenous TERRA mols. in live cells. Here, we developed a method to visualize TERRA in live cells via a combination of CRISPR Cas13 RNA labeling and SunTag technol. Single-particle tracking reveals that TERRA foci undergo anomalous diffusion in a manner that depends on the timescale and telomeric localization. Furthermore, we used a chem.-induced protein dimerization system to manipulate TERRA subcellular localization in live cells. Overall, our approaches to monitor and control TERRA locations in live cells provide powerful tools to better understand its roles in telomere maintenance and genomic integrity.
Frontiers in Molecular Biosciences published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (TERRA). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Application In Synthesis of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics