Wang, Lan; Xian, Yan-Fang; Hu, Zhen; Loo, Steven King Fan; Ip, Siu Po; Chan, Wood Yee; Lin, Zhi-Xiu; Wu, Justin Che Yuen published the artcile< Efficacy and action mechanisms of a Chinese herbal formula on experimental models of atopic dermatitis>, Synthetic Route of 112-63-0, the main research area is Chinese herbal formula exptl model atopic dermatitis; Atopic dermatitis; Chinese herbal formula; Epidermal barrier; NF-κB pathway; Skin inflammation; Th1/Th2 balance.
Atopic dermatitis (AD) is a skin inflammatory disease characterized by erythema, eruption, lichenification and pruritus. Shi Zhen Formula (SZF), an empirical Chinese herbal preparation, has clin. efficacy in relieving the symptoms of AD patients. However, the underlying mol. mechanisms of SZF remained unclear. We aimed to investigate the anti-AD effects of SZF and elucidate its underlying mol. mechanisms using in vitro and in vivo models of AD. High-performance liquid chromatog. anal. was performed for quality control of SZF extract The anti-inflammatory effect of SZF was investigated through evaluating the levels of nitric oxide (NO), chemokines and pro-inflammatory cytokines in the lipopolysaccharide (LPS) stimulated RAW264.7 cells. AD-like skin lesions in female BALB/c mice were induced by 2,4-dinitrochlorobenzene (DNCB). SZF (3.15, 6.30 and 9.45 g/kg) and dexamethasone (5 mg/kg) were administered by gavage daily for 15 consecutive days. The body weight, skin thickness, skin dermatitis severity and scratching behaviors were recorded throughout the study. Histol. anal., reverse transcription-quant. polymerase chain reaction (RT-PCR), western blot (WB) and ELISA anal. were used to illuminate the mol. targets associated with the anti-AD effects of SZF. SZF markedly decreased the epidermal thickening and infiltration of mast cells in the ears and dorsal skin of the 2,4-dinitrochlorobenzene (DNCB)-treated mice. SZF not only suppressed the levels of IgE (IgE), histamine, thymic stromal lymphopoietin (TSLP) and IL-4 in the serum but also suppressed the over-production of IL-4 and IL-6 and gene expressions of IL-4, IL-13, IL-31 and TSLP in the dorsal skin. Moreover, SZF improved epidermal barrier by increasing the protein expressions of filaggrin, involucrin and loricrin and inhibited the activation of NF-κB p65 pathway in the dorsal skin of the DNCB-treated mice. SZF alleviates DNCB induced AD-like skin lesions in mice through regulating Th1/Th2 balance, improving epidermal barrier and inhibiting skin inflammation. Our research findings provide scientific footing on the use of this Chinese herbal formula for the treatment of AD.
Journal of Ethnopharmacology published new progress about Atopic dermatitis. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Synthetic Route of 112-63-0.
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