Jitian, Simion; White, Samuel R.; Yang, H.-H. Wendy; Weisz, Adrian published the artcile< Conventional high-performance liquid chromatography versus derivative spectrophotometry for the determination of 1,3,6-pyrenetrisulfonic acid trisodium salt and 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt in the color additive D&C Green Number 8 (Pyranine)>, SDS of cas: 112-63-0, the main research area is pyranine Green 8 P3S P4S derivative spectrophotometry HPLC; 1,3,6,8-Pyrenetetrasulfonic acid sodium salt; 1,3,6-Pyrenetrisulfonic acid sodium salt; D&C Green Number 8; Derivative spectrophotometry; HPLC; Pyranine.
Specifications in the U.S. Code of Federal Regulations for the color additive D&C Green Number 8 (Color Index Number 59040) limit the levels of the subsidiary colors 1,3,6-pyrenetrisulfonic acid trisodium salt (P3S) and 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt (P4S). The present paper describes a comparative study of two possible methods to replace the currently used multi-step TLC/spectrophotometry method of separating and quantifying the minor components P3S and P4S in G8. One of the new approaches uses conventional high-performance liquid chromatog. (HPLC) and the other, derivative spectrophotometry. While the derivative spectrophotometric method was shown to be inadequate for the anal. of minor components overwhelmed by components of much higher concentration, the HPLC method was proven highly effective. The closely related, very polar compounds P3S and P4S were separated by the new HPLC method in less than 4 min using a conventional HPLC instrument. P3S and P4S were quantified by using five-point calibration curves with data points that ranged from 0.45 to 7.63% and from 0.13 to 1.82%, by weight, for P3S and P4S, resp. The HPLC method was applied to the anal. of test portions from 20 batches of D&C Green Number 8 submitted to the U.S. Food and Drug Administration for certification.
Journal of Chromatography A published new progress about Derivative spectrophotometry. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, SDS of cas: 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics