Bayer, Edward A.; Viswanatha, T.; Wilchek, Meir published the artcile< The use of a homologous series of affinity labeling reagents in the study of the biotin transport system in yeast cells>, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is biotin homolog yeast uptake; transport biotin homolog yeast; affinity label biotin transport.
A homologous series of 9 biotin-containing affinity labels, in which the reactive p-nitrophenyl ester group is located at increasing distances from the biol.-active ureido moiety, were synthesized. Cells, harvested from growth cultures of Saccharomyces cerevisiae, were suspended in K phosphate solution (pH 4.0) and treated with an EtOH solution containing 1 of the affinity labeled homologs. The cells then were washed, resuspended in phosphate solution containing glucose, and biotin-14C was added. Aliquots were taken at intervals, the cells collected by filtration, and the radioactivity was determined The percent inhibition of biotin uptake was greatest with the shorter-length affinity labels, and the inhibition decreased with increasing chain length. With some exceptions, the inhibited cells could be reactivated by adding mercaptoethanol. The relation of the structure of the homologs to the mechanism of biotin transport was discussed.
FEBS Letters published new progress about Biological transport. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Safety of (9Z,12Z)-Methyl octadeca-9,12-dienoate.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics