Reference of Methyl SalicylateIn 2019 ,《Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance》 was published in Scientific Reports. The article was written by Lima Silva, Caio Cesar de; Shimo, Hugo Massayoshi; de Felicio, Rafael; Mercaldi, Gustavo Fernando; Rocco, Silvana Aparecida; Benedetti, Celso Eduardo. The article contains the following contents:
Salicylic acid (SA) and its Me ester, Me salicylate (MeSA) are well known inducers of local and systemic plant defense responses, resp. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homolog protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Mol. modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, Whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria. The experimental part of the paper was very detailed, including the reaction process of Methyl Salicylate(cas: 119-36-8Reference of Methyl Salicylate)
Methyl Salicylate(cas: 119-36-8) is a natural herbivore-induced plant volatile. It is a naturally occurring product in trees, legumes, exotic plants, vegetables, berries, and the primary constituent of the oil of wintergreen.Methyl Salicylate is produced from salicylic acid.Reference of Methyl Salicylate
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