Shivalingam, Arun; Taemaitree, Lapatrada; El-Sagheer, Afaf H.; Brown, Tom published the artcile< Squaramides and Ureas: A Flexible Approach to Polymerase-Compatible Nucleic Acid Assembly>, Computed Properties of 112-63-0, the main research area is squaramide ureas flexible nucleic acid assembly; RNA detection; ligation; nucleic acids; polymerase chain reaction; squaramide.
Joining oligonucleotides together (ligation) is a powerful means of retrieving information from the nanoscale. To recover this information, the linkages created must be compatible with polymerases. However, enzymic ligation is restrictive and current chem. ligation methods lack flexibility. Herein, a versatile ligation platform based on the formation of urea and squaramide artificial backbones from minimally modified 3′- and 5′-amino oligonucleotides is described. One-pot ligation gives a urea linkage with excellent read-through speed, or a squaramide linkage that is read-through under selective conditions. The squaramide linkage can be broken and reformed on demand, while stable pre-activated precursor oligonucleotides expand the scope of the ligation reaction to reagent-free, mild conditions. The utility of the authors’ system is demonstrated by replacing the enzymically biased RNA-to-DNA reverse transcription step of RT-qPCR with a rapid nucleic-acid-template-dependent DNA chem. ligation system, that allows direct RNA detection.
Angewandte Chemie, International Edition published new progress about DNA Role: ARG (Analytical Reagent Use), BUU (Biological Use, Unclassified), SPN (Synthetic Preparation), ANST (Analytical Study), USES (Uses), BIOL (Biological Study), PREP (Preparation). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Computed Properties of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics