《Exploring adipose tissue structure by methylsalicylate clearing and 3D imaging》 was written by Gilleron, Jerome; Meziat, Cindy; Sulen, Andre; Ivanov, Stoyan; Jager, Jennifer; Esteve, David; Muller, Catherine; Tanti, Jeanfrancois; Cormont, Mireille. COA of Formula: C8H8O3 And the article was included in Journal of Visualized Experiments in 2020. The article conveys some information:
Obesity is a major worldwide public health issue that increases the risk to develop cardiovascular diseases, type-2 diabetes, and liver diseases. Obesity is characterized by an increase in adipose tissue (AT) mass due to adipocyte hyperplasia and/or hypertrophia, leading to profound remodeling of its three-dimensional structure. Indeed, the maximal capacity of AT to expand during obesity is pivotal to the development of obesity-associated pathologies. This AT expansion is an important homeostatic mechanism to enable adaptation to an excess of energy intake and to avoid deleterious lipid spillover to other metabolic organs, such as muscle and liver. Therefore, understanding the structural remodeling that leads to the failure of AT expansion is a fundamental question with high clin. applicability. In this article, we describe a simple and fast clearing method that is routinely used in our laboratory to explore the morphol. of mouse and human white adipose tissue by fluorescent imaging. This optimized AT clearing method is easily performed in any standard laboratory equipped with a chem. hood, a temperature-controlled orbital shaker and a fluorescent microscope. Moreover, the chem. compounds used are readily available. Importantly, this method allows one to resolve the 3D AT structure by staining various markers to specifically visualize the adipocytes, the neuronal and vascular networks, and the innate and adaptive immune cells distribution.Methyl Salicylate(cas: 119-36-8COA of Formula: C8H8O3) was used in this study.
Methyl Salicylate(cas: 119-36-8) is a natural herbivore-induced plant volatile. It is a naturally occurring product in trees, legumes, exotic plants, vegetables, berries, and the primary constituent of the oil of wintergreen.Methyl Salicylate is produced from salicylic acid.COA of Formula: C8H8O3
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