Srankova, Maria; Dvorak, Ales; Martinek, Marek; Sebej, Peter; Klan, Petr; Vitek, Libor; Muchova, Lucie published the artcile< Antiproliferative and Cytotoxic Activities of Fluorescein-A Diagnostic Angiography Dye>, Category: esters-buliding-blocks, the main research area is fluorescein diagnostic angiog dye antiproliferative cytotoxicity cancer human; carbon monoxide; fluorescein; irradiation; metabolism; proliferation; singlet oxygen; viability.
Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic mols., such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these mols. can affect physiol. processes, the main aim of this study was to explore the potential biol. impacts of fluorescein photochem. In our in vitro study in a human hepatoblastoma HepG2 cell line, we explored the possible effects on cell viability, cellular energy metabolism, and the cell cycle. We observed markedly lowered cell viability (&30%, 75-2400μM) upon irradiation of intracellular fluorescein and proved that this decrease in viability was dependent on the cellular oxygen concentration We also detected a significantly decreased concentration of Krebs cycle metabolites (lactate and citrate < 30%; 2-hydroxyglutarate and 2-oxoglutarate < 10%) as well as cell cycle arrest (decrease in the G2 phase of 18%). These observations suggest that this photochem. reaction could have important biol. consequences and may account for some adverse reactions observed in fluorescein-treated patients. Addnl., the biol. activities of both 1O2 and CO might have considerable therapeutic potential, particularly in the treatment of cancer. International Journal of Molecular Sciences published new progress about Angiography imaging agents. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Category: esters-buliding-blocks.
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