Gao, Ziqing; Mi, Rui; Cheng, Zhaoxi; Li, Xiaofeng; Zeng, Huawu; Wu, Gaosong; Zhao, Jing; Zhang, Weidong; Ye, Ji published the artcile< Integrated metabolomics and network pharmacology revealed Hong-Hua-Xiao-Yao tablet's effect of mediating hormone synthesis in the treatment of mammary gland hyperplasia>, Formula: C19H34O2, the main research area is HHXYT hormone synthesis mammary gland hyperplasia metabolomics network pharmacol; Hong-Hua-Xiao-Yao tablet; mammary gland hyperplasia; metabolomics; network pharmacology; pharmacological effects; traditional medicine.
Hong-Hua-Xiao-Yao Tablet (HHXYT) is a traditional Chinese medicine (TCM) formula that has been approved for the treatment of mammary gland hyperplasia (MGH), but its mechanism of action is unclear. In this study, a strategy that integrated metabolomics and network pharmacol. was applied to systemically reveal the mechanism of HHXYT in the treatment of MGH. Our pharmacodynamic study indicated that the proliferation of mammary gland was inhibited in rats, and serum-level disorder of estradiol and progesterone was reversed after HHXYT treatment. 54 compounds absorbed in rat plasma were identified after administration of HHXYT. The serum metabolome revealed 58 endogenous differential metabolites, of which 31% were steroid lipids metabolites, with steroid hormone biosynthesis being the most significant metabolic module. 7 targets, 6 herbs, and 17 ingredients were found to play key roles in HHXYT’s treatment of MGH. 3 of the 7 key targets (CYP11A1, HSD3B2, and CYP17A1) were directly involved in androgen synthesis, while 2 targets (AR and ESR1) were receptors for the direct action of androgens and estrogens. Mol. docking was utilized to confirm the bindings between the 5 targets and their corresponding compounds In an in vitro test, HHXYT (50μg/mL) and its ingredient formononetin (3.2, 6.3, and 12.5μM) were found to significantly reduce the increase of testosterone level induced by dexamethasone (10μM) in thecal cells. In summary, this study illustrated that the mechanism of HHXYT’s treatment of MGH was to regulate hormone disorder. HHXYT could reduce estrogen-stimulated hyperplasia by inhibiting the production of its precursor androgen.
Frontiers in Pharmacology published new progress about Androgen receptors Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Formula: C19H34O2.
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