Wang, Tong; Guo, Qing; Yang, Hua; Gao, Wen; Li, Ping published the artcile< pH-controlled reversible deep-eutectic solvent based enzyme system for simultaneous extraction and in-situ separation of isoflavones from Pueraria lobata>, Formula: C19H34O2, the main research area is Pueraria deep eutectic solvent extraction isoflavones.
In this study, we developed an efficient and green pH-controlled reversible deep-eutectic solvents (DESs) based enzyme system for efficient extraction, transformation and in-situ separation of natural products from herbs. As a case study, seven isoflavones in the root of Pueraria lobata were selected as target compounds A series of DESs were firstly prepared and the extraction efficiency for target compounds with or without cellulase catalysis was investigated. We found that [N4444][Cl]:ethylene glycol (1:2) with cellulase addition show the highest extraction efficiency for isoflavones. More importantly, the phase behavior of [N4444][Cl]:ethylene glycol (1:2) based cellulase system was found to be pH-controlled. When pH is 5.0, the system is a homogeneous single-phase system, which is conducive to the extraction of natural products. When pH is 6.4, the system changes from the single-phase to the heterogeneous two-phase, to realize the in-situ separation of the compounds Therefore, by adjusting the pH of the system, the in-situ separation of isoflavones was successfully realized with high recovery yields. The pH-controlled DESs based enzyme-assisted extraction system overcomes the incompatibility between enzyme catalysis and conventional organic solvent in extraction, which could efficiently extract seven isoflavones from Pueraria lobata root.
Separation and Purification Technology published new progress about Deep eutectic solvents. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Formula: C19H34O2.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics