Garcia de Jalon, Elvira; Ruiz de Garibay, Gorka; Haug, Bengt Erik; McCormack, Emmet published the artcile< CytoCy5S, a compound of many structures. in vitro and in vivo evaluation of four near-infrared fluorescent substrates of nitroreductase (NTR)>, SDS of cas: 112-63-0, the main research area is nitroreductase fluorescent probe imaging.
CytoCy5S, a quenched, red-shifted fluorescent probe, has been used to exploit the imaging potential of the nitroreductase (NTR) reporter gene platform. Its use has been reported in a number of publications, however there are discrepancies in both the reported structure and its physicochem. properties. Herein, we aim to highlight these discrepancies and to define the best candidate of the four substrates under study for preclin. work in NTR reporting by optical applications. We report the synthesis, purification and characterization of four NTR substrates, including alternately described structures currently referred by the name CytoCy5S. A comparative NTR enzymic assay was performed to assess the spectroscopic characteristics of the different reductively activated probes. The NTR expressing triple-neg. breast carcinoma cell line, MDA-MB-231 NTR+, was employed to compare, both in vitro and in vivo, the suitability of these fluorescent probes as reporters of NTR activity. Comparison of the reporting properties was achieved by flow cytometry, fluorescence microscopy and optical imaging, both in vivo and ex vivo. This study evaluated the different spectroscopic and biol. characteristics of the four substrates and concluded that substrate 1 presents the best features for oncol. in vivo preclin. optical imaging.
Dyes and Pigments published new progress about Fluorescence imaging. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, SDS of cas: 112-63-0.
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