Liang, Jesse; Sohrabi, Alireza; Epperson, Mary; Rad, Laila M.; Tamura, Kelly; Sathialingam, Mayilone; Skandakumar, Thamira; Lue, Philip; Huang, Jeremy; Popoli, James; Yackly, Aidan; Bick, Michael; Wang, Ze Zhong; Chen, Chia-Chun; Varuzhanyan, Grigor; Damoiseaux, Robert; Seidlits, Stephanie K. published the artcile< Hydrogel arrays enable increased throughput for screening effects of matrix components and therapeutics in 3D tumor models>, Reference of 112-63-0, the main research area is hydrogel array throughput screening matrix component therapeutics tumor model.
Cell-matrix interactions mediate complex physiol. processes through biochem., mech., and geometrical cues, influencing pathol. changes and therapeutic responses. Accounting for matrix effects earlier in the drug development pipeline is expected to increase the likelihood of clin. success of novel therapeutics. Biomaterial-based strategies recapitulating specific tissue microenvironments in 3D cell culture exist but integrating these with the 2D culture methods primarily used for drug screening has been challenging. Thus, the protocol presented here details the development of methods for 3D culture within miniaturized biomaterial matrixes in a multi-well plate format to facilitate integration with existing drug screening pipelines and conventional assays for cell viability. Since the matrix features critical for preserving clin. relevant phenotypes in cultured cells are expected to be highly tissue- and disease-specific, combinatorial screening of matrix parameters will be necessary to identify appropriate conditions for specific applications. The methods described here use a miniaturized culture format to assess cancer cell responses to orthogonal variation of matrix mechanics and ligand presentation. Specifically, this study demonstrates the use of this platform to investigate the effects of matrix parameters on the responses of patient-derived glioblastoma (GBM) cells to chemotherapy.
Journal of Visualized Experiments published new progress about Biological materials. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Reference of 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics