Lesley, Scott A.; Patten, Phillip A.; Schultz, Peter G. published the artcile< A genetic approach to the generation of antibodies with enhanced catalytic activities>, COA of Formula: C19H34O2, the main research area is catalytic antibody recombinant hydrolase activity; mol cloning gene hydrolytic catalytic antibody; Escherichia hydrolytic catalytic antibody expression selection; genetic selection hydrolytic catalytic antibody.
A hydrolytic catalytic antibody, generated against a nitrophenyl phosphonate transition state analog, has been cloned and expressed in Escherichia coli for use as a model system to demonstrate the feasibility of using genetic selections to enhance catalytic activity. Conditions were found that permit the secretion of active recombinant antibody into the periplasm of a strain of E. coli deficient in the biotin biosynthetic genes (Δbio-gal). A number of substrates were synthesized that, upon hydrolysis by the antibody, yield free biotin, which is required for cell growth. The substrates and selections can be used to identify mutants of the antibody with altered activities. This approach should be generalizable to a wide number of hydrolytic reactions including the selective cleavage of peptide, polysaccharide, phosphodiester, and ester bonds.
Proceedings of the National Academy of Sciences of the United States of America published new progress about Animal gene Role: PRP (Properties). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, COA of Formula: C19H34O2.
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